Highlights Pristine river ecosystem hosts ESBL ARGs, like blaTEM and blaCTX-M. The Yamuna River hosts multidrug, fluoroquinolone, fosfomycin, and β-lactam resistance genes. Predominance of biofilm-forming bacteria and their co-occurrence with the ARGs. Abstract Freshwater ecosystems are important reservoirs and transmission pathways for antibiotic resistance genes (ARG), yet host-mediated microbial selection and anthropogenic pressure on fish gut resistome remain poorly chara

Highlights Qdr3 coordinates cellular homeostasis in C. auris. qdr3 deletion remodels mitochondrial function. Loss of Qdr3 alters biofilm architecture and extracellular matrix production. qdr3 deletion enhances virulence. Qdr3 links membrane transport, mitochondrial function, and virulence. Abstract Candida auris (also known as Candidozyma auris) relies on extensive transporter networks to maintain cellular homeostasis and adapt to environmental stress, yet the broader regulat

Highlights C. albicans Th17 cells are restricted to a few proteins contained in fungal EVs The oral mucosa is a major reservoir of Th17 cells, dominated by C. albicans reactivity C. albicans-reactive Th17 cells are shared between oral and gut tissues Homeostatic C. albicans Th17 cells adapt pathogenic traits in Crohn’s disease Summary The commensal yeast Candida albicans is a major inducer of human mucosal Th17 cells. How C. albicans drives Th17 cell responses at homeostasis,

Highlights Single-cell Raman spectroscopy coupled with D2O labeling was first applied to Vibrio splendidus persisters. V. splendidus persisters are metabolically active rather than fully dormant. Persisters show remodeled cellular components with decreased nucleic acids and increased lipids and polysaccharides. Vitamin C inhibits persisters by suppressing the stringent response, providing a green anti-persister approach. Abstract Persister cells, a multidrug-tolerant bacteria