Highlights Single-cell Raman spectroscopy coupled with D2O labeling was first applied to Vibrio splendidus persisters. V. splendidus persisters are metabolically active rather than fully dormant. Persisters show remodeled cellular components with decreased nucleic acids and increased lipids and polysaccharides. Vitamin C inhibits persisters by suppressing the stringent response, providing a green anti-persister approach. Abstract Persister cells, a multidrug-tolerant bacteria

Highlights Sublethal oxytetracycline and CuCl2 elevated per-cell 18S rDNA/rRNA copy numbers, disrupting allometric scaling with cell volume in two ciliated protists. Oxytetracycline selectively increased rRNA: rDNA ratios, revealing stressor-specific ribosomal regulation. Both stressors induced persistent intragenomic polymorphisms of 18S rRNA gene. Pollutant-induced ASV inflation may bias microbial diversity estimates. Abstract Ribosomal RNA (rRNA) genes serve as foundationa

Highlights Bedtime probiotic intake retains 100,000x more bacteria than daytime intake. Oral-origin L. reuteri strain colonizes better than the non-oral isolate. Probiotic persistence varies widely interindividually, some individuals retain strains 7+ days. Supragingival plaque shows highest proportional probiotic abundance by site. Higher probiotic levels correlate with greater pocket depth reduction in periodontitis. Abstract The clinical application of probiotics for oral

Highlights The global regulator ppGpp plays a central role in controlling AIEC virulence. Loss of ppGpp impairs virulence across in vitro and in vivo infection models. Two candidate biomarkers with discriminatory potential for AIEC identification were defined. Abstract Strain LF82 belongs to the Adherent-Invasive Escherichia coli (AIEC) pathotype, found in 21% to 63% of Crohn’s disease (CD) patients. Many studies have explored its specific virulence mechanisms, but AIEC isola